So, they can provide excellent sensitivity combined with superior specificity and reproducibility, only available from a recombinant antibody. Recombinant multiclonal antibodies are a defined mixture of carefully selected individual recombinant monoclonal antibodies designed to recognize different epitopes on the same antigen. For applications where a polyclonal antibody would traditionally be used (eg, when analyzing low-abundance targets or detecting multiple post-translational modifications at once), recombinant multiclonal antibodies can offer an ideal solution. We recommend using recombinant monoclonal antibodies when a suitable clone exists for your particular target and application to ensure experimental reproducibility and long-term antibody supply. Since the antibody-encoding sequence is known and defined, it can be further engineered and manipulated for its intended use. Compared to traditional monoclonal and polyclonal antibodies, recombinant antibodies offer long-term, secured supply with a minimal batch-to-batch variation. The term 'recombinant' refers to antibodies produced in vitro using synthetic genes. Monoclonal antibodies have high specificity for their target, low non-specific cross-reactivity, and minimal batch-to-batch variations. In contrast to polyclonal antibodies, monoclonal antibodies only recognize a single epitope per antigen. However, they are limited in supply, subject to high batch-to-batch variability, and exhibit cross-reactivity and lack of specificity. By binding to several different epitopes, polyclonal antibodies can produce a strong signal against the target antigen in their relevant application and are not biased against a single epitope. To recap, polyclonal antibodies consist of a heterogeneous mixture of antibodies, with each antibody recognizing different epitopes of a particular antigen. These antibodies have distinct advantages and limitations covered here. When choosing a primary antibody, consider the following: Antibody clonality and manufacture methodĬlonality is determined by whether the antibodies come from different B-cells (polyclonal antibodies) or identical B-cells derived from a parent clone (monoclonal antibodies). A primary antibody is an antibody that binds directly to a target protein, with a variable antibody region recognizing a protein's epitope.
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